2020年土壤氮含量对豇豆根瘤固氮酶活性、根瘤干重和作物生长的影响数据库

于2020年4月-6月在河北省农林科学院农业资源环境研究所网室内进行培养试验，1个品种、5个氮水平，收获时剪掉地上部之前先挖取豆角根系，清洗后选取大小一致、大于2 mm直径的根瘤2颗，吸干水分放入西林瓶，塞紧胶塞，用注射器抽取1 mL空气，加入1 mL乙炔气体，用封口膜封好，反应2 h，将混合气体打入空瓶，用于测定根瘤固氮酶活性。共采集植株（地上部+地下部）样品80个，根瘤样品40个，冲掉泥土吸净水分称鲜重、杀青烘干数据。A culture experiment was conducted in the screen house of the Institute of Agricultural Resources and Environment, Hebei Academy of Agriculture and Forestry Sciences from April to June 2020. The experiment utilized one bean cultivar and five nitrogen application levels. Prior to harvesting and removing the aboveground plant parts, the root systems of the bean plants were excavated. After rinsing to remove adhering soil, two root nodules with consistent size and a diameter greater than 2 mm were selected, blotted dry to eliminate surface moisture, and placed into glass serum vials. The rubber stoppers were tightly fitted, then 1 mL of air was withdrawn from the vial headspace using a syringe, and 1 mL of acetylene gas was injected into the vial. The vial was sealed with Parafilm and incubated for 2 hours. The resulting gas mixture was then transferred to an empty vial for the determination of root nodule nitrogenase activity. In total, 80 plant samples (consisting of both aboveground and underground tissues) and 40 root nodule samples were collected. All samples were rinsed free of soil, blotted dry, weighed for fresh weight, then subjected to high-temperature fixation and oven drying to acquire dry weight measurement data.