GLs .xlsx

Glucosinolate content was analyzed using 4 weeks old plants. Five plants of each line were used as biological replicates. Plants were cut from top of the soil, put in liquid nitrogen to stop chemical reactions and transferred to freeze-drier for 3 days. Freeze-dried samples were finely ground using micro-dismemberator U (B. Braun Biotech International). Finely grounded material was sent to NIOO for dedicated glucosinolate analysis. Briefly, samples were boiled in 70% MeOH followed by washing two times in 1 ml of 70% MeOH and two times with 1 mL MQ water and one times in 2mL of NaOAC 16 hr ahead of treatment with 20 μl arylsulfatase. Following arylsulfatase treatment freeze dried elute was redissolved in 1mL MQ water and filtered over a 20 μm filter. Separation and detection of glucosinolates were performed using reversed phase C-18 column and photodiode array detector respectively. Glucosinolates measurement and concentration calculation was done following the procedure of Kabouw et al., (2010). A list of measured glucosinolates and their abbreviated names are given in本研究以4周龄植株为材料分析硫代葡萄糖苷（glucosinolate）含量。每个品系选取5株植株作为生物学重复。将植株从土壤顶端处剪断，置于液氮中终止体内化学反应，随后转移至冷冻干燥机中冻干3天。使用微粉碎机U（B. Braun Biotech International）对冷冻干燥后的样品进行精细研磨。将研磨得到的样品送至NIOO开展专属硫代葡萄糖苷检测分析。简要流程如下：样品先在70%甲醇溶液中煮沸，随后依次用1 mL 70%甲醇溶液洗涤2次、1 mL超纯水洗涤2次，再以2 mL乙酸钠溶液浸泡16小时，之后加入20 μL芳基硫酸酯酶进行酶解反应。酶解完成后，将冻干洗脱液重新溶解于1 mL超纯水中，经20 μm滤膜过滤。硫代葡萄糖苷的分离与检测分别采用反相C-18色谱柱与光电二极管阵列检测器完成。硫代葡萄糖苷的含量测定与浓度计算参照Kabouw等人（2010）的实验流程完成。本研究测定的硫代葡萄糖苷及其缩写名称详见[原文未完整呈现后续内容]。