Hypolipidemic and antioxidant effects of the juice and water seed extracts of two pomegranate species in high-cholesterol diet fed rats

Abstract This study investigated the hypolipidemic and antioxidant effects of the juice and water seed extracts of two pomegranate species from Saudi Arabia and Egypt, and compared them with each other as well as with ellagic acid (EA) in high-cholesterol diet (HCD) fed rats. Compared with the case in rats fed HCD alone, EA and juice or water seed extracts of both pomegranate types significantly reduced the levels of total cholesterol, triglycerides, LDL-c, and GSH, increased the level of HDL-c, increased the activity of SOD and CAT, and decreased the level of MDA in the serum of HCD-fed rats. There were more profound effects of the juice or water seed extract of both the Saudi and Egyptian pomegranates on serum HDL-c and GSH levels and the activities of SOD and CAT than those of EA. In conclusion, this study showed that water seed extracts or juice of both types of pomegranate ameliorated HCD-induced hyperlipidemia, lipid peroxidation, and changes of enzymatic and non-enzymatic antioxidants in the serum of HCD-fed rats.

摘要 本研究探讨了来自沙特阿拉伯与埃及的两种石榴的果汁及水提种子提取物的降血脂与抗氧化功效，并将其与彼此以及鞣花酸（ellagic acid，EA）在高胆固醇饮食（high-cholesterol diet，HCD）喂养的大鼠中进行对比。与仅喂食高胆固醇饮食的大鼠相比，两种石榴的果汁或水提种子提取物以及鞣花酸均可显著降低高胆固醇饮食喂养大鼠血清中的总胆固醇、甘油三酯、低密度脂蛋白胆固醇（low-density lipoprotein cholesterol，LDL-c）、谷胱甘肽（glutathione，GSH）水平，同时升高高密度脂蛋白胆固醇（high-density lipoprotein cholesterol，HDL-c）水平，增强超氧化物歧化酶（superoxide dismutase，SOD）与过氧化氢酶（catalase，CAT）活性，并降低丙二醛（malondialdehyde，MDA）水平。相较于鞣花酸，沙特与埃及两种石榴的果汁或水提种子提取物对大鼠血清高密度脂蛋白胆固醇、谷胱甘肽水平以及超氧化物歧化酶、过氧化氢酶活性的改善效果更为显著。综上，本研究表明，两种石榴的水提种子提取物或果汁均可改善高胆固醇饮食诱导的大鼠高脂血症、脂质过氧化以及血清中酶性与非酶性抗氧化物质的异常变化。