Maternal gut microbiota in pregnancy influences obesity in offspring
收藏NIAID Data Ecosystem2026-03-11 收录
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GF IQI mice were housed in vinyl isolators under a 12-h light-dark cycle, and given regular chow.On day 19, pregnant GF or SPF IQI mice were delivered by caesarean section, and the foetuses were born by conventional foster mice for 4 weeks. Fecal samples of offsprings was collected during lactation and in adulthood.Sequencing of the 16S rRNA variable regions 3 and 4 was performed as described previously. The libraries were sequenced using Miseq system (Illumina) with a 2 * 300 base-pair protocols.
创建时间：
2020-07-19
