Purpose : We performed strand-specific RNA-seq analysis of CHO cells according to adaptation trajectory to identify the specific genes responded to the suspension adaptation. Result : Cell growth, rep
Our research was focused to the evaluation of Sodium Acetate (NaAc) in the HEK293 cell culture. HEK293 is employed for the stable production of the extracellular domain HER1 at the Center of Molecular
The cultures of R27T were established in a shake flask and 7.5 L (working volume: 5 L), or 30 L (working volume: 20 L) bioreactors. For ELISA, R27T was obtained from bioreactors on day 7, or from shak
CHO cells grown in serum-free medium have an absolute requirement for putrescine supplementation due to their deficiency in activity of the enzyme arginase. Consequently, principally in this system, p
