CK-3B

CK-3B investigates the impact of Bifidobacterium bifidum treatment on the gut microbiota in Clostridioides difficile infection. Fecal samples from mice post-infection were processed using Invitrogen with sequencing on the Ion Torrent NGS System. Analysis via Ion Reporter SW (16S) revealed shifts in microbial taxa dynamics over days 1, 3, and 6, shedding light on the potential of probiotics in modulating gut microbiota during C. difficile infection.The identification of the sample CK-3B:<br>C: Denotes the strain ST-2 of Clostridioides difficile.<br>K: Represents timepoint 3, indicating sampling after treatment.<br>3: Refers to the specific mouse number under investigation.<br>B: Indicates the treatment administered, which in this case is Bifidobacterium bifidum.CK-3B 旨在探究两歧双歧杆菌（Bifidobacterium bifidum）干预对艰难梭菌（Clostridioides difficile）感染小鼠肠道菌群的影响。研究人员对感染建模后的小鼠粪便样本采用Invitrogen相关试剂盒进行前处理，并通过Ion Torrent 下一代测序（Next Generation Sequencing, NGS）系统完成测序。借助Ion Reporter SW（16S）开展分析后，研究团队观察到感染后第1、3、6天的微生物类群动态变化，为揭示益生菌在艰难梭菌感染期间调控肠道菌群的潜在价值提供了依据。样本CK-3B的命名规则如下：<br>C代表所使用的艰难梭菌菌株ST-2；<br>K代表采样时间点，即干预后的采样节点；<br>3代表本次研究中的受试小鼠编号；<br>B代表施加的干预方式，即本次实验所使用的两歧双歧杆菌。