Plasmodium 3D7 Genome Assembly With PacBio Data and CLEAR
收藏资源简介:
This fileset contains data, code and IPython Notebooks demostrating<br>how to assemble the hard to assemble 80% AT-rich Plasmodium 3D7 genome. The first thing you might want to do is probably to read through<br>''P3D7_CLEAR_NOTE.pdf'' to know what this dataset is about. You will have to "gunzip" the two main data file ''preads.fa.gz'' and<br>''pr_pr_strigent.m4.gz'' to use them. The IPython Notebook ''P3D7_CLEAR_NOTE.ipynb'' and ''P3D7_CLEAR_NOTE_P2.ipynb''<br>can be run to generate the assembly if you have proper computation environment. There are also the PDF verison of the notebooks, ''P3D7_CLEAR_NOTE.pdf''<br>and ''P3D7_CLEAR_NOTE_P2.pdf''. Some requirement on how to reproduce the<br>computational experiment can be found in ''P3D7_CLEAR_NOTE.pdf''.
本数据集包含实验数据、代码与IPython笔记本(IPython Notebooks),用于演示如何组装难以组装的AT碱基占比达80%的高AT富集型疟原虫(Plasmodium)3D7基因组。研究者可首先参阅「P3D7_CLEAR_NOTE.pdf」以了解本数据集的相关背景与内容。如需使用两个核心数据文件,需先通过gunzip工具分别解压「preads.fa.gz」与「pr_pr_strigent.m4.gz」。若研究者具备适配的计算环境,可运行IPython笔记本「P3D7_CLEAR_NOTE.ipynb」与「P3D7_CLEAR_NOTE_P2.ipynb」以完成基因组组装流程。本数据集同时提供上述两款笔记本的PDF版本:「P3D7_CLEAR_NOTE.pdf」与「P3D7_CLEAR_NOTE_P2.pdf」。有关复现本次计算实验的具体要求,均可在「P3D7_CLEAR_NOTE.pdf」中查阅到。



