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Preliminary screening of HOB and MOB co-cultivations

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Figshare2021-08-06 更新2026-04-08 收录
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This is the preliminary screening for Kerckhof, Sakarika <i>et al</i> 2021 - the project where this dataset belongs to and the accompanying paper. In this set 9 MOB and the 19 HOB obtained from an isolation campaign were combined (1 MOB with 1 HOB) and grown in liquid medium in order to obtain the best performing combinations. The experiments were performed in 96-well plates, at a final working volume of 0.2 mL. The MOB and HOB pure cultures were diluted in DSMZ medium 81 containing trace elements, at a final cell density of 10<sup>6 </sup>cells/mL. Next a volume of 0.2 mL was transferred in the respective well for the growth of the individual strains, while for the combinations of one HOB and one MOB, a volume of 0.1 mL and from each strain was used (i.e. to achieve a final volume of 0.2 mL). Each combination was tested in duplicate

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2021-08-06
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