Dataset_Dengue_Ptricornutum

Dengue, caused by the dengue virus (DENV), is a global health threat transmitted by <i>Aedes</i> mosquitoes, resulting in 400 million cases annually. The disease ranges from mild to severe, with potential progression to hemorrhagic dengue. Current research is focused on natural antivirals due to challenges in vector control. This study evaluates the antiviral potential of peptides derived from the microalgae <i>Phaeodactylum tricornutum</i>, known for its bioactive compounds. The microalgae were cultivated under controlled conditions, followed by protein extraction and hydrolysis to produce four peptide fractions. These fractions were assessed for cytotoxicity via the MTT assay and antiviral activity against DENV serotype 2 using flow cytometry and plaque formation assays. The 10-30 kDa peptide fraction, at 150 and 300 μg/ml concentrations, demonstrated no cytotoxicity and significantly reduced the percentage of infected cells and viral titers. These findings suggest that peptides from <i>Phaeodactylum tricornutum</i> exhibit promising antiviral activity against dengue virus serotype 2, potentially contributing to developing new therapeutic approaches for dengue.登革热（Dengue）由登革病毒（Dengue Virus, DENV）引发，是一种经伊蚊属（Aedes）蚊虫传播的全球公共卫生威胁，每年造成约4亿例感染病例。该疾病临床表现从轻症到重症不等，可进展为登革出血热。当前由于媒介防控存在诸多挑战，相关研究聚焦于天然抗病毒制剂的开发。本研究评估了源自三角褐指藻（Phaeodactylum tricornutum）的多肽的抗病毒潜力，该微藻以其丰富的生物活性化合物而闻名。研究人员在可控培养条件下扩增该微藻，随后通过蛋白提取与水解步骤制备得到四种多肽组分。采用MTT法对各组分的细胞毒性进行检测，并通过流式细胞术与空斑形成实验，评估其对抗登革病毒2型血清型（DENV serotype 2）的抗病毒活性。在150 μg/ml与300 μg/ml的给药浓度下，分子量为10-30 kDa的多肽组分未表现出细胞毒性，且可显著降低感染细胞比例与病毒滴度。上述研究结果表明，三角褐指藻来源的多肽对登革病毒2型血清型展现出极具潜力的抗病毒活性，有望为登革热新型治疗策略的开发提供新思路。