The contribution of monocyte-derived dendritic cells (DC) to an immune response is essential for the elimination of pathogens. In vitro DC can be generated by treatment of monocytes with GM-CSF + IL-4
In this study, the glutamine synthetase gene and fut8 gene were simultaneously knocked out in CHO cells with CRISPR/Cas 9 technology and homologous replacement.
Transcription activator-like (TAL) effector nucleases (TALENs) have enabled the introduction of targeted genetic alterations into a broad range of cell lines and organisms. These customizable nuclease