The Enzyme Kinetics Module from SigmaPlot 10 was used to evaluate the inhibition type and Ki values. A detailed statistical report was created for each binding model to compare different models and to
The ecotin-MASP affinities in Table 1 were measured at thermodynamic equilibrium as described in the Materials and methods. For tight-binding inhibition assays, enzyme and inhibitor concentrations nee
The following trajectories were employed in the generation of MSM models and water analyses: SaFabI_60us_align.zip EcFabI_60us_align.zip waters_SaFabI.tar.gz waters_EcFabI.tar.gz
This Excel file contains initial velocity data for product formation in enzyme inhibition experiments. These datasets are used to generate the figures presented in the manuscript and supplementary not
