+, all tested strains showed measurable enzyme activity;–, no tested strain showed measurable enzyme activity; +/–, 50% or more tested strains showed measurable enzyme activity;–/+, 50% or less showed
aThe enzyme activity was measured using DNS method.bThe enzyme activity was measured using iodine method, thus the result can’t be directly compared with those measured using DNS method.NM: Not mentio
Fig. 3BCells expressing the His-Xpress tagged MIPS were grown in SC medium to the early stationary phase. Enzymatic activity of MIPS protein purified from cell extracts was determined.
