Conservation of the residues involved in bpDNase I ion binding sites across four DNase I families in various species.

The bpDNase I sequence was aligned with 30 non-redundant DNase I, 16 DNase I-like 1, 11 DNase I-like 2 and 17 DNase I-like 3 sequences from different species. I (%) corresponds to the percentage of identity for each of the amino acids involved in each of the four bpDNase I cation binding sites, I, II, III and IVa,b. For relevant cases, S (%) is the percentage of the amino acid or the type of amino acid substituting those of bpDNase I.