<b>Metabolomic Analysis Reveals the Mechanisms Underlying Ischemic Stroke Induced by Vitamin B12 Deficiency</b>

<b>Objective: </b>This study aims to explore the impact of vitamin B12 deficiency on the pathogenesis of ischemic stroke through a comprehensive analysis combining metabolomics and proteomics methodologies.<b>Methods: </b>Patients experiencing their first episode of acute ischemic stroke (AIS) will be recruited and categorized into two groups based on the presence or absence of vitamin B12 deficiency, alongside a matched healthy control cohort. An extensive untargeted metabolomic investigation will be conducted using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) to detect differentially expressed metabolites (DEMs) among the groups. The protein expression profiles will be analyzed using 4D-DIA proteomics technology to identify significantly differentially expressed proteins (DEPs). Subsequently, an integrated analysis of the identified DEMs and DEPs will be performed to unveil potential biomarkers and metabolic pathways implicated in the pathogenesis of ischemic stroke.**研究目的：** 本研究旨在通过代谢组学与蛋白质组学相结合的综合分析手段，探究维生素B12缺乏对缺血性脑卒中发病机制的影响。**研究方法：** 招募首次发生急性缺血性脑卒中（acute ischemic stroke, AIS）的患者，根据是否存在维生素B12缺乏将其分为两组，并设置匹配的健康对照队列。采用超高效液相色谱-质谱（ultra-performance liquid chromatography-mass spectrometry, UPLC-MS）开展大规模非靶向代谢组学研究，以检测各组间的差异表达代谢物（differentially expressed metabolites, DEMs）；采用4D-DIA蛋白质组学技术分析蛋白质表达谱，筛选得到显著差异表达蛋白质（differentially expressed proteins, DEPs）。随后对上述差异表达代谢物与差异表达蛋白质进行整合分析，以揭示与缺血性脑卒中发病机制相关的潜在生物标志物及代谢通路。