Determination of kinetic parameters for mucilage derived glycosyl hydrolases.
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Experiments were run in triplicate under the optimal pH and temperature for each enzyme. Both the enzyme concentration and 4NP substrates concentration varied over ranges of 8–20 μM and 0.2 to 1mM respectively. All reactions were carried out in the corresponding buffer (either citrate-phosphate or phosphate) with a final buffer concentration of 50 mM.
创建时间：
2018-09-26
