Gene expression alterations of pancreatic intraepithlial neoplasia. Homo sapiens

Gene expression data from the RNA sequencing of laser microdissected pancreatic intraepithelial neoplasia and pancreatic normal ducts. Overall design: All RNA samples were isolated from laser microdissected tissues. Samples were prepared in two sets. 13 samples (1 normal duct, 2 PanIN-1s, 3 PanIN-2s, and 7 PanIN-3s) were sequenced on an ABI SOLiD 5500 instrument. 8 samples (2 normal duct, 2 PanIN-1s, 2 PanIN-2s, and 2 PanIN-3s) were sequenced on an Illumina HiSeq instrument. Datasets were combined using ComBat batch removal.本数据集为激光显微切割（laser microdissection）获取的胰腺上皮内瘤变（pancreatic intraepithelial neoplasia, PanIN）与正常胰腺导管的RNA测序基因表达数据。实验设计：所有RNA样本均提取自激光显微切割的组织。样本分为两组制备：第一组包含13个样本（1例正常导管、2例PanIN-1、3例PanIN-2及7例PanIN-3），在ABI SOLiD 5500测序仪上完成测序；第二组包含8个样本（2例正常导管、2例PanIN-1、2例PanIN-2及2例PanIN-3），在Illumina HiSeq测序仪上完成测序。最终采用ComBat批次校正方法对两组数据集进行合并整合。