Conductive bacterial cellulose by <i>in situ</i> laccase polymerization of aniline

Conductive and colored bacterial cellulose (BC) was developed by entrapment of polyaniline (PANi) onto dry BC membranes. The polyaniline was produced by in situ green polymerization of aniline by Myceliophthora thermophila laccase at pH = 4, 25°C, in the presence of a mediator, 1-hydroxybenzotriazol (HBT), using two different reactors, a water bath (WB) and an ultrasonic bath (US). MALDI-TOF and 1H NMR characterization of the experiment solutions confirmed the efficient polymerization of aniline by laccase. The dried BC membranes with entrapped polyaniline showed electrical conductive behavior and strong coloration, opening novel routes for the exploitation of functionalized bacterial cellulose as a green material for technical textiles, wearables, and other applications.本研究通过将聚苯胺（polyaniline, PANi）包埋于干燥细菌纤维素（bacterial cellulose, BC）膜上，制备得到导电型有色细菌纤维素。该聚苯胺由嗜热毁丝霉漆酶（Myceliophthora thermophila laccase）在pH=4、25℃条件下，以1-羟基苯并三唑（1-hydroxybenzotriazol, HBT）为介体，原位绿色聚合苯胺制得，实验采用水浴（water bath, WB）与超声浴（ultrasonic bath, US）两种反应器开展。对反应溶液进行基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）与氢核磁共振波谱（1H NMR）表征后，证实漆酶可高效催化苯胺聚合。负载聚苯胺后的干燥细菌纤维素膜兼具导电性能与强着色能力，为功能化细菌纤维素作为绿色材料应用于功能性纺织品、可穿戴设备及其他相关领域开辟了全新路径。