HAEC-T Cell Coculture Flow Cytometry

HAEC treated with IFNg 24hr, then cocultured with allogeneic PBMC for 24hr. Both nonadherent and adherent fractions stained for CD3 PE CD4 violetFluor 450 CD8a FITC IL-2Ra APC CD69 PE-Cy7 CD105 BV510将人类主动脉内皮细胞（Human Aortic Endothelial Cells，HAEC）用干扰素γ（Interferon gamma，IFNg）处理24小时，随后与同种异体外周血单个核细胞（allogeneic Peripheral Blood Mononuclear Cells，PBMC）共培养24小时。对非贴壁组分与贴壁组分分别进行如下染色：CD3 PE、CD4 violetFluor 450、CD8a FITC、IL-2Ra APC、CD69 PE-Cy7 及 CD105 BV510。