Self-delivering CRISPR RNAs for AAV Co-delivery and Genome Editing in vivo

Our proof-of-concept establishment of AAV crRNA co delivery offers a route towards transient editing activity, target multiplexing, guide redosing, and vector inactivation.本研究开展的腺相关病毒（AAV）与CRISPR RNA（crRNA）共递送概念验证体系构建，为实现瞬时编辑活性、多靶点编辑、向导RNA重复递送以及载体失活提供了可行路径。