Ferlapioside: a new bromine substituted iridoid glycoside from the roots of <i>Ferula lapidosa</i> Korovin

One new bromine substituted iridoid glycoside, ferlapioside (1), was isolated from the roots of Ferula lapidosa Korovin, together with 10 known ones including seven iridoid glycosides (2–8) and three sesquiterpene lactones (9–11). Their structures were elucidated by extensive spectroscopic analyses, e.g. 1D and 2D-NMR, HR-ESI-MS, IR, UV, CD techniques, and comparison with literature values. Compounds 1 and 3–11 were evaluated for inhibitory activity against LPS-induced NO production in the RAW 264.7 cells and the in vitro cytotoxic activity against five human cancer cell lines (HL-60, A549, SMMC-7721, MDA-MB-231 and SW480). However, none of them exhibit significant anti-inflammatory and cytotoxic activity. This study will provide a scientific basis and reference for the further development and utilisation of Ferula plants.本研究从硬阿魏（Ferula lapidosa Korovin）的根中分离得到1个新的溴代环烯醚萜苷（iridoid glycoside）类化合物ferlapioside（1），同时获得10个已知化合物，其中包括7个环烯醚萜苷（2~8）及3个倍半萜内酯（sesquiterpene lactones，9~11）。所有化合物的结构均通过一维核磁共振波谱（1D-NMR）、二维核磁共振波谱（2D-NMR）、高分辨电喷雾电离质谱（HR-ESI-MS）、红外光谱（IR）、紫外光谱（UV）及圆二色谱（CD）等多种波谱分析手段，并结合文献数据比对得以解析。对化合物1及3~11开展了脂多糖（LPS）诱导的RAW 264.7细胞一氧化氮（NO）生成抑制活性评价，以及针对5种人癌细胞系（HL-60、A549、SMMC-7721、MDA-MB-231与SW480）的体外细胞毒活性评价。结果表明，所有受试化合物均未表现出显著的抗炎及细胞毒活性。本研究可为阿魏属植物的后续开发与利用提供科学依据与参考。