Quantitative analysis of super-resolution imaging data on late endosome/lysosome heterogeneity.

This study develops a multiplexed and quantitative DNA-PAINT super-resolution imaging pipeline to investigate the distribution of late endosomal/lysosomal (LEL) proteins across individual LELs, revealing cell-type specific LEL sub-populations with unique protein compositions, offering insights into organelle heterogeneity at single-organelle resolution.本研究开发了一套多路定量DNA-PAINT超分辨率成像流程，以探究单个晚期内体/溶酶体（late endosomal/lysosomal, LEL）内晚期内体/溶酶体蛋白的分布特征，揭示了具备独特蛋白组成的细胞类型特异性LEL亚群，为单细胞器分辨率下的细胞器异质性研究提供了重要见解。