DOCK1 insufficiency disrupts trophoblast function and pregnancy outcomes via DUSP4-ERK pathway

To investigate the role of DOCK1 in trophoblast regulation, we established HTR-8 lines in which DOCK1 has been knocked out by sgRNA using CRISPR/Cas9. Comparative gene expression profiling analysis of RNA-seq data for DOCK1-KO and its control in HTR-8 cells.为探究DOCK1在滋养层细胞调控中的作用，我们利用CRISPR/Cas9系统，通过单向导RNA（single guide RNA，sgRNA）构建了DOCK1基因敲除的HTR-8细胞系；随后对该基因敲除组（DOCK1-KO）及其对照组的HTR-8细胞的RNA测序（RNA-seq）数据开展比较基因表达谱分析。