Differences in miRNA Detection Levels are Technology and Sequence Dependent [Agilent array]. Homo sapiens

We undertook an integrative technological approach to compare miRNA detection capability of three high-throughput commercial platforms. Overall design: We artificially introduced human precursor, 2’-O-methyl modified and mature spiked-in miRNAs in a controlled fashion into native human placenta total RNA.本研究采用整合式技术方案，对比三种高通量商业化平台的微小RNA（miRNA）检测性能。实验设计概述：本研究以可控方式，将人类前体、经2’-O-甲基修饰以及成熟的掺入型微小RNA引入天然人类胎盘总RNA中。