Mammalian core histones were identified as DEN2 C binding partners.

The 100 amino acid mature DEN2 C protein fused to streptavidin binding protein and calmodulin binding protein (CBP) tags was expressed in Huh7 liver cells and interactions identified using a tandem affinity purification assay. Proteins that bound tagged DEN2 C were isolated and identified by LC-MS/MS spectrometry.将带有链霉亲和素结合蛋白（streptavidin binding protein）与钙调蛋白结合蛋白（calmodulin binding protein，CBP）标签的100个氨基酸残基的成熟登革病毒2型（DEN2）C蛋白在Huh7肝癌细胞中表达，并通过串联亲和纯化实验（tandem affinity purification assay）鉴定其互作蛋白。结合带标签DEN2 C蛋白的蛋白质经分离后，采用液相色谱-串联质谱联用技术（LC-MS/MS spectrometry）完成鉴定。