Supplementary Information files for Rapid quantification of prion proteins using resistive pulse sensing

Supplementary Information files for Rapid quantification of prion proteins using resistive pulse sensing<br>Prion diseases are a group of fatal transmissible neurological conditions caused by the change in conformation of intrinsic cellular prion protein (PrPC). We present a rapid assay using aptamers and resistive pulse sensing, RPS, to extract and quantify PrPC from complex sample matrices. We functionalise the surface of superparamagnetic beads, SPBs, with a DNA aptamer. First SPB’s termed P-beads, are used to pre-concentrate the analyte from a large sample volume. The PrPC protein is then eluted from the P-beads before aptamer modified sensing beads, S-beads, are added. The velocity of the S-beads through the nanopore reveals the concentration of the<br>《利用电阻脉冲传感快速定量朊蛋白》的补充信息文件<br>朊病毒病是一类致命的传染性神经系统疾病，由内源性细胞型朊蛋白（PrPC）的构象变化引起。我们提出一种基于适配体和电阻脉冲传感（resistive pulse sensing，RPS）的快速检测方法，用于从复杂样本基质中提取并定量PrPC。我们使用DNA适配体对超顺磁珠（superparamagnetic beads，SPBs）的表面进行功能化修饰。首先，将超顺磁珠（SPBs）标记为P-珠，用于从大体积样本中预浓缩分析物。随后，从P-珠上洗脱PrPC蛋白，再加入适配体修饰的传感珠（S-珠）。S-珠通过纳米孔的速度可反映出（PrPC的）浓度。