Figure 1B - PbANKA + EPR10411 - Raw Image Files

Raw image files to: Figure 1. Localisation of Ago2 to the iRBC. (A) IFA of P. falciparum 3D7 blood stages. Yellow: Ago2 (EPR10411), magenta: PfTubulin. (B) IF staining of P. berghei ANKA rings and trophozoites. Yellow: Ago2 (EPR10411), magenta: PbHsp70. Nuclei were stained with Hoechst (blue). Images were taken on a Nikon spinning disc confocal microscope (100x objective). Representative images of at least 5 per condition are shown. Controls using only a secondary antibody revealed no unspecific staining. Scale bar indicates 5 µm.本数据集配套的原始图像文件如下： 图1 Ago2在感染红细胞（iRBC）中的定位。(A) 恶性疟原虫（P. falciparum）3D7株红内期的间接免疫荧光试验（IFA）：黄色荧光信号标记Ago2（EPR10411），品红色荧光信号标记PfTubulin。(B) 伯氏疟原虫（P. berghei）ANKA株环状体与滋养体的间接免疫荧光染色：黄色荧光信号标记Ago2（EPR10411），品红色荧光信号标记PbHsp70；细胞核经Hoechst染色（蓝色）。所有图像均采用Nikon转盘式共聚焦显微镜（100倍物镜）采集。每个实验条件下均展示至少5张代表性图像。仅使用二抗的对照实验未观测到非特异性染色。比例尺为5 µm。